Intergeneric somatic hybridization pdf

Intergeneric asymmetric somatic hybrids have been obtained by the fusion of metabolically inactivated protoplasts from embryogenic suspension cultures ofFestuca arundinacea (recipient) and protoplasts from a non-morphogenic cell suspension ofLolium multiflorum (donor) irradiated with 10, 25, 50, 100, 250 and 500 Gy of X-rays. Regenerating calli led to the recovery of genotypically and In this paper, we focus on somatic hybridization between Citrus species and P. trifoliata or F1 sexual intergeneric hybrids between Citrus and Poncirus, such as citranges, citrumelos, and citrandarins. The results of three new combinations of somatic hybridization are presented. Summary. Intergeneric symmetric and asymmetric somatic hybrids have been obtained by fusion of metabolically inactivated protoplasts from embryogenic suspension cultures of tall fescue (Festuca arundinacea Schreb.) and unirradiated or 10-500 Gy-irradiated protoplasts from non-morphogenic cell suspensions of Italian ryegrass (Lolium multiflorum Lam.)..). Genotypically and phenotypically 35 Citations Metrics Abstract An intergeneric somatic hybrid was obtained upon fusion of protoplasts of rice and barley. Protoplasts isolated from suspension cultures of rice cells were fused by electrofusion with protoplasts that had been isolated from young barley leaves. Some of the resultant calli formed green spots and shoots. Interspecific hybridization is the crossing of two species from the same genus. This allows the exploitation of useful genes from wild, unimproved species for the benefit of the cultivated species. The Pisum species are diploid self-pollinators sharing a similar karyotype. Asymmetric Somatic Plant Hybridization: Status and Applications. American Journal of Plant Sciences. Tom Eeckhaut. Prabhu Lakshmanan. Johan Van Huylenbroeck. Erik Van Bockstaele. Dieter Deryckere. Download Download PDF. Full PDF Package Download Full PDF Package. This Paper. A short summary of this paper. 37 Full PDFs related to this paper. Read Paper. Download Download PDF. Download Full PDF Regeneration of plants from Ipomoea cairica L. protoplasts and production of somatic hybrids between I. cairica L. and sweetpotato, I. batatas (L.) Lam By Jimin Guo Cell Differentiation in Shoot Meristem: A Molecular Perspective Abstract An intergeneric somatic hybrid was obtained upon fusion of protoplasts of rice and barley. Protoplasts isolated from suspension cultures of rice cells were fused by electrofusion with protoplasts that had been isolated from young barley leaves. Some of the resultant calli formed green spots and shoots. Intergeneric symmetric and asymmetric somatic hybrids have been obtained by fusion of metabolically inactivated protoplasts from embryogenic suspension cultures of tall fescue ( Festuca arundinacea Schreb.) and unirradiated or 10-500 Gy-irradiated protoplasts from non-morphogenic cell suspensions of Italian ryegrass ( Lolium multiflorum Lam.). The application of plant tissue culture, through protoplast culture and somatic hybridization, has been used to induce genetic variability and to create new nuclear—cytoplasmic combinations in several plant species.29 However, the efficient use of these techniques is subject to the achievement of complete plant regeneration. Tzvel - ScienceDirect Plant Science Volume 120, Issue 2, 4 November 1996, Pages 197-203 Plant regeneration from intergeneric somatic hybridizati