Nikon a1 confocal manual

The Nikon Imaging Centre at King's College London (NIC@King's) is a core facility for light microscopy developed in partnership between King's College London and Nikon Instruments UK. A1 upright confocal. High resolution imaging - The A1+ utilizes a galvano scanner which enables high-resolution imaging of up to 4096 x 4096 pixels and up RCF Nikon Confocal & SIM User Manual. Publications using N-SIM Super Resolution Microscope System . Nikon Confocal and superresolution microscopy e-book (Science/AAAS Publishing group) Nikon Microscopy Education Objectives. Obj. Objective. Medium. Use. 10x. Plan Apo 10x/0.45NA. Dry. Confocal. 20x. Confocal Nikon a1R Spectral Nikon A1r spectral Imaging live (short time-lapses <2 hours) and ˜xed samples - any ˜uorescent proteins Depth (z) Speed acq. Resolution Bleaching Multicolor high good medium 4-8 <30 um Temperature, Humidity, CO2 control Peristaltic pum, water bath 3D Z-stacks, tiled images Colocalization, F-techniques (FRAP, FRET,) CONFOCAL PLATFORM (Nikon A1R-HD, 2018): 4 diode Lasers (405 nm, 488nm, 561 nm, 640 nm) 4 PMTS (Standard: Blue and Far Red, GaASP: Green and Red) 32 channel spectral detector. Transmitted light Detector for Differential Interference Contrast (DIC) microscopy. NIS Elements Advanced software v 5.2 The Nikon A1R is a point scanning confocal microscope based around a fully motorized, inverted Nikon Ti body with hardware-enabled focus correction. The Nikon A1R has three photomultipliers for fluorescence imaging, one for transmitted light detection and a dedicated photomultiplier array for spectral profiling or if required, unmixing of Home | Biomedical Research Core Facilities, University of Michigan The Nikon A1si is a confocal system equipped with a point-scan scan head, 5 standard PMT detectors and a 32-channel PMT spectral detector. The A1si's highest resolution mode is up to 4096 x 4096 Nikon A1/TiE Training Guide Prepared for University of Houston 2015 . 2 is manual; the mag changer must match what is set on the microscope Confocal Acquisition: Quick Start *The A1 Simple GUI is a wizard that will walk you through acquiring a confocal image 1. Make sure you have previewed your slide through the eyepieces and focused on This was the beginning of a revolutionary line of confocal microscopes that would span years and help bring Confocal microscopy to labs all over the world. This system, combined with Nikon's flagship TE2000 inverted microscope, boasted an ultra-compact, state of the art multi-channel laser confocal with an easy to use software interface all Nikon A1R FLIM Confocal Microscope Location: 1-220H CCRB Features include Nikon's patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 20x water immersion, 40x oil, and 60x oil magnifications for DIC. 1. First, set your confocal settings as needed for imaging. 2. Open the "A1 Stimulation" and "ND Stimulation" dialog boxes under View Acquistion Controls on the top toolbar. 3. Set up as shown below. Select your bleaching laser (405) and adjust power (usually 100%) 4. The ND experiment should be saved to file (choose your location).