Pglo instructor manual

Student Manual pGLO Transformation Lesson 1 Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. This protein gives an organism a particular trait. An instructor's manual, available through McGraw-Hill Lab Central, provides detailed advice based on the authors' experience on how to prepare materials for each lab, teachings tips and lesson plans, and questions that can be used in quizzes and practical exams. This manual is an excellent Student Manual pGLO Transformation Student Manual pGLO Transformation Lesson 1 Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. Student Manual pGLO Transformation - WordPress.com Page 4/9 Instructors Guide Pglo Transformation With the pGLO Transformation Kit, students use a simple procedure to transform bacte- ria with a gene that codes for a Green Fluorescent Protein (GFP). The real-life source of this gene is the bioluminescent jellyfish Aequorea victoria. The gene codes for a Green Using pGLO to transform bac teria, students can actually Manual poses a series of questions to focus and stimulate thinking about all aspects of the investigation. Answers are provided in the Instructor's Answer Guide. Student involvement in this process will result in an increased understanding of the It will certainly ease you to look guide Pglo Transformation Lab Teacher Manual Ap Biology as you such as. By searching the title, publisher, or authors of guide you really want, you can discover them rapidly. In the house, workplace, or perhaps in your method AP® Biology framework while allowing significant flexibility for instructors. Answers are provided in the Instructor's Answer Guide. Student involvement in this process will result in an increased understanding of the scientific process and the value of proceeding into a task in an organized and logical fashion. Chapter 12 - BIO 121 Manual_pGLO-2.docx. City Colleges of Chicago, Malcolm X. BIO 121. Biology; Bacteria; In this experiment, both (-) pGLO plates are control plates. The LB/amp control plate can be compared to the LB/amp (+)pGLO plate. This comparison shows that genetic transformation produces bacterial colonies that can grow on ampicillin (due to the uptake of the pGLO plasmid and the expression of the ampicillin resistance gene). The (-) pGLO/LB 100% (1 rating) 5. In (+pGLO) LB + Ampicillin plate the bacteria will grow but not glow. The pGLO plasmid contains gene that provide resistance against ampicillin and an another gene that codes for GFP protein. But the expression of GFP coding gene depends on arabin …. View the full answer. Label one closed micro test tube +pGLO and another -pGLO . Label both tubes with your group's name. Place them in the foam tube rack. 2. Open the tubes and, using a sterile transfer pipet, transfer 250 µl of transformation solution (CaCl 2 ) into each tube. 33 +pGLO +pGLO -pGLO -pGLO Transformation Solution 250 μl 3. Place the tubes on ice. 4.